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Configuration of the 2PLM imaging setup with an LC-SLM. M1- M7: mirrors; HWP: half-wave plate; P: polarizer; EOM: electro-optic modulator; SH: shutter; PH: pinhole; L1 - L4: lenses; LC-SLM: liquid crystal spatial light modulator; IR: iris diaphragm; GM: galvo mirrors; SL: scan lens; TL: tube lens; DM: dichroic mirror; OL: objective lens (20X); EFs: emission filters; PMTs: photomultiplier tubes; CAM: CMOS camera. The vortex mask (topological charge m = 5 used as an example) is combined with a blazed grating mask when projected onto the LC-SLM to separate the torus focal spot (1 st order) from the 0 th order of LC-SLM diffraction.

Journal: Biomedical Optics Express

Article Title: Out-of-focus signal rejection for in vivo pO 2 measurements using two-photon phosphorescence lifetime microscopy

doi: 10.1364/BOE.532084

Figure Lengend Snippet: Configuration of the 2PLM imaging setup with an LC-SLM. M1- M7: mirrors; HWP: half-wave plate; P: polarizer; EOM: electro-optic modulator; SH: shutter; PH: pinhole; L1 - L4: lenses; LC-SLM: liquid crystal spatial light modulator; IR: iris diaphragm; GM: galvo mirrors; SL: scan lens; TL: tube lens; DM: dichroic mirror; OL: objective lens (20X); EFs: emission filters; PMTs: photomultiplier tubes; CAM: CMOS camera. The vortex mask (topological charge m = 5 used as an example) is combined with a blazed grating mask when projected onto the LC-SLM to separate the torus focal spot (1 st order) from the 0 th order of LC-SLM diffraction.

Article Snippet: The MATLAB-based control software of 2PLM used in the work is also available online [ ].

Techniques: Imaging

Examples of in vivo phosphorescence lifetime measurements for intravascular pO 2 from various locations in pial arterioles and venules obtained using 2PLM. (a) A survey image was acquired to select pO 2 imaging locations. Two locations (1 and 2) were selected in arterioles, and the other two locations (3 and 4) were selected in venules. Scale bar: 100 µm. P.C.: Photon counts. (b) Phosphorescence decays (dotted lines) acquired from the selected four locations in (a) with 2000 laser excitation cycles, and their corresponding lifetime and pO 2 values.

Journal: Biomedical Optics Express

Article Title: Out-of-focus signal rejection for in vivo pO 2 measurements using two-photon phosphorescence lifetime microscopy

doi: 10.1364/BOE.532084

Figure Lengend Snippet: Examples of in vivo phosphorescence lifetime measurements for intravascular pO 2 from various locations in pial arterioles and venules obtained using 2PLM. (a) A survey image was acquired to select pO 2 imaging locations. Two locations (1 and 2) were selected in arterioles, and the other two locations (3 and 4) were selected in venules. Scale bar: 100 µm. P.C.: Photon counts. (b) Phosphorescence decays (dotted lines) acquired from the selected four locations in (a) with 2000 laser excitation cycles, and their corresponding lifetime and pO 2 values.

Article Snippet: The MATLAB-based control software of 2PLM used in the work is also available online [ ].

Techniques: In Vivo, Imaging